Science & Technology

New technology will improve sequencing capabilities and enable us to decipher the genomes of all life on Earth.

The Earlham Institute (EI) uses a twin set of state-of-the-art Pacific Biosciences Sequel IIe platforms to support the EarthBioGenome project to enhance the capabilities of high-fidelity long read sequences and biodiversity genomics to the UK bioscience community. We provide important technology for. ..

The Earth BioGenome Project (EBP) is gaining momentum to sequence, catalog, and characterize the biodiversity genomes of all eukaryotes on Earth within the next decade, resulting in a large number of highs. Global efforts are underway to deploy the technologies and infrastructure that we can provide to. -High quality genomic sequence.

The Sequel IIe platform enables scientists to take genomic analysis to a higher level Accuracy By generating fidelity long reads (HiFi) to solve the genome and transcriptome.

“The Sequel IIe platform allows us to scale up our existing infrastructure in contributing to the BioGenome sequence,” said Dr. Karim Gharbi, Head of Genomics Pipelines at the Earlham Institute. “The demand for high-quality genomic references from the UK bioscience community is growing rapidly, and the demand for access to HiFi sequence data is higher than ever.

Earlham Institute Sequel IIe Platform

The sequel IIe platform of the Earlham Institute. Credit: Earlham Institute (EI)

“Feedback from early adopters of Sequel IIe across the genomics community is very positive for the HiFi genome, at least an order of magnitude higher than existing resources. Additional platforms increase the capacity of genomic sequencing capabilities. It will soon double and give EI researchers and British bioscientists access to HiFi leads in a continuous and cost-effective manner. “

Over the past few years, the Earlham Institute has made strategic investments in genome-enabled technology, paving the way for a new era of increasingly standard biology, with high-quality, annotated genome sequences no longer an exception. ..

Professor Neil Hall of the Earlham Institute said: “The EarthBioGenome project initiative is very supportive, but we need to ensure the technology and infrastructure to generate high-quality genomes on a large scale. The Earlham Institute’s additional HiFi capabilities for analyzing protozoan genomes are available. , Strengthens our position in the Global Initiative as a Leader in Genome Sequencing. “

These sequencing technologies support several national and international initiatives with the Earlham Institute as a core research partner. – – Includes Vertebrate Genome Project, Darwin Tree of Life (DTOL) Project, and European Reference Genome Atlas (ERGA) – – It provides key sequence data and analysis of a variety of organisms and underpins an ambitious program to catalog the biodiversity of unicellular eukaryotes (protists). *

“The key to this investment was the early adoption of a long, high-quality (HiFi) sequencing platform (Pacific Biosciences Sequel II) in 2019 before the Earlham Institute acquired the equipment permanently with BBSRC funding. That was it, “added Dr. Gharbi. “This technology allowed our researchers to quickly succeed in providing high-quality genomic references for major target species, establishing EI as a key center for BioGenome research. “


* As part of the Welcome-sponsored DToL project, the Earlham Institute is also leading the development of new tools for building high-quality genomes from single-cell eukaryotes. At the heart of this technology is a platform and pipeline of prokaryotic species isolated from environmental populations, increasing efficiency and reducing the cost of library preparation from individual cells.

New technology will improve sequencing capabilities and enable us to decipher the genomes of all life on Earth. New technology will improve sequencing capabilities and enable us to decipher the genomes of all life on Earth.

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